Chloroquine cell culture

Discussion in 'Canadian Pharmacy' started by Ardis, 29-Feb-2020.

  1. promovah Moderator

    Chloroquine cell culture


    No effective prophylactic or post-exposure therapy is currently available. We report, however, that chloroquine has strong antiviral effects on SARS-Co V infection of primate cells.

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    In a three-page paper published in Cell Research, scientists at the Wuhan Institute of Virology’s State Key Laboratory of Virology wrote that both Chloroquine and the antiviral Remdesivir were, individually, “highly effective” at inhibiting replication of the novel coronavirus in cell culture. Although the cause of this discrepancy is not clear, differences in cell source and culture conditions might be responsible for the different results. Collectively, our result showed that TNF-α mRNA is not decreased by chloroquine either in primary cells or in monocytic transformed cells. Jan 27, 2010 Chloroquine CQ, traditionally used as an antimalarial medication, has emerged as a prospective adjunct in tumor therapy. CQ has been found to strongly potentiate the inhibitory effect of radiation on cancer cell proliferation, presumably via negative regulation of DNA repair processes.

    In addition to the well-known functions of chloroquine such as elevations of endosomal p H, the drug appears to interfere with terminal glycosylation of the cellular receptor, angiotensin-converting enzyme 2. These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage.

    Chloroquine cell culture

    How long is chloroquine half life when I treat a cell line., Chloroquine inhibits production of TNF-α, IL-1β and IL-6 from.

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  7. I have tried daily change with medium with new chloroquine for thje autophagy studies and keep the same culture medium and chloroquine for 24-72 hours so far I have not seen difference in the results.

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    The conclusion that chloroquine is a zinc ionophore is based on the detection of significantly elevated intracellular zinc levels when both zinc and chloroquine were added to the cell culture medium. The fluorescent zinc probe used in this study is a well-established intracellular marker for zinc ions and has been validated in previous studies. Inhibition of Zika virus ZIKV infection by chloroquine in Vero cells. A Vero cells were infected with ZIKV MR766 at a multiplicity of infection MOI of 2, treated with chloroquine for 5 days, and were then stained for the viral envelope protein and. Chloroquine is an inhibitor of the lysosomal degradation of the DNA which is taken up by the cells, so as leelee said, transfection should have been successful, albeit at a slightly lower level than if you would have added the chloroquine. In my normal transfections, I never add chloroquine and get high efficiencies nevertheless.

     
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