Confocal immunofluorescent analysis of He La (upper) and C2C12 (lower) cells, chloroquine-treated (50 μM, overnight; left), nutrient-starved with EBSS (3 hr, middle) or untreated (right) using LC3A/B (D3U4C) XP Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using LC3A/B (D3U4C) XP® Rabbit m Ab in the presence of control peptide (left) or antigen-specific peptide (right). Western blot analysis of extracts from He La cells, mock transfected or transfected with rat LC3B, and from HT-1080 and A20 cells, untreated or chloroquine-treated (50 μM, overnight), using LC3B Antibody. Hydroxychloroquine nutrient interactions Scarring alopecia plaquenil dosage Can a patient on plaquenil get the varicella vaccine Plaquenil lyme disease Programmed cell death10. it is considered to be an alter-native tumor-suppressing mechanism induced by genomic instability, suppression of cell growth, and degradation of Chloroquine promotes gefitinib‑induced apoptosis by inhibiting protective autophagy in cutaneous squamous cell carcinoma Chloroquine is an antimalarial drug that has been used in humans for many years. In recent years, Chloroquine has been shown to inhibit autophagy and induce apoptosis in malignant cells and thus has been tested in various experimental model systems and in human clinical trials. Chloroquine LC3B-I HT-1080 A20 60 50 40 20 10 – – – +– HeLa mock +LC3B Western blot analysis of extracts from HeLa cells, mock transfected or transfected with rat LC3B, and from HT-1080 and A20 cells, untreated or chloroquine-treated 50 µM, overnight, using LC3B Antibody. Entrez-Gene ID # 81631 Swiss-Prot Acc. # Q9GZQ8 Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Confocal immunofluorescent analysis of HCT-116 cells, untreated (left) or choroquine-treated (50 u M, overnight; right) using LC3B Antibody (green) and β-Catenin (L54E2) Mouse m Ab (Alexa Fluor® 555 Conjugate) #5612 (red). Chloroquine cell signalling A chloroquine-induced macrophage-preconditioning strategy for., Chloroquine Is a Zinc Ionophore - PubMed Central PMC Can hydroxychloroquine cause staphylococcus aureus uti Chloroquine can also act on the immune system through cell signalling and regulation of pro-inflammatory cytokines. Chloroquine is known to inhibit phosphorylation activation of the p38 mitogen-activated protein kinase MAPK in THP-1 cells as well as caspase-1. New insights on the antiviral effects of chloroquine against.. LC3B Antibody - Cell Signaling Technology. Where and How Chloroquine Fights Cancer Science Signaling. An antibody shouldn’t be one of the variables in your experiment. Find out why customers rank CST highest for antibody specificity and sensitivity. When imaging using a confocal microscope there is no clear LC3 signal, and definitely no LC3 positive punctae as you would expect to see. I treated some cell lines with 25 mM Chloroquine for. Chloroquine is a lysosomotropic agent that prevents endosomal acidification 1. It accumulates inside the acidic parts of the cell, including endosomes and lysosomes. This accumulation leads to inhibition of lysosomal enzymes that require an acidic pH, and prevents fusion of endosomes and lysosomes.